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Catalog Number Size Price
LS-B10260-50 50 µl (0.72 mg/ml) $460 
SERPINA1 / Alpha 1 Antitrypsin Antibody - Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
SERPINA1 / Alpha 1 Antitrypsin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
SERPINA1 / Alpha 1 Antitrypsin Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.
SERPINA1 / Alpha 1 Antitrypsin Antibody - Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 5B12, IHC, WB) LS‑B10260

IHC‑plus™ Monoclonal Mouse anti‑Human SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 5B12, IHC, WB) LS‑B10260

Note: This antibody replaces LS-C114480
Antibody:
SERPINA1 / Alpha 1 Antitrypsin Mouse anti-Human Monoclonal (5B12) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Monkey, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B10260-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SERPINA1 / Alpha 1 Antitrypsin Mouse anti-Human Monoclonal (5B12) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Monkey, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha 1 Antitrypsin antibody LS-B10260 is an unconjugated mouse monoclonal antibody to Alpha 1 Antitrypsin (SERPINA1) from human. It is reactive with human, dog and monkey. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SERPINA1 / Alpha 1 Antitrypsin
Synonyms
SERPINA1 | A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | PI1 | Serpin A1 | Alpha 1 AT
Host
Mouse
Reactivity
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
5B12
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Full length human recombinant protein of human SERPINA1 (NP_000286) produced in HEK293T cell.
Specificity
Human SERPINA1 / Alpha 1 Antitrypsin
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINA1 / Alpha 1 Antitrypsin
P01009 K02212 AAB59495.1

Validation

SERPINA1 / Alpha 1 Antitrypsin Antibody - Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 5B12) for IHC, WB/Western, IP LS-B10260 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024