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| Catalog Number | Size | Price |
|---|---|---|
| LS-B6516-50 | 50 µl (1 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human RTN4 / Nogo Antibody (aa14‑30, IHC, WB) LS‑B6516
IHC‑plus™ Polyclonal Rabbit anti‑Human RTN4 / Nogo Antibody (aa14‑30, IHC, WB) LS‑B6516
Note: This antibody replaces LS-C19342
Antibody:
RTN4 / Nogo Rabbit anti-Human Polyclonal (aa14-30) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
RTN4 / Nogo Rabbit anti-Human Polyclonal (aa14-30) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
Nogo antibody LS-B6516 is an unconjugated rabbit polyclonal antibody to Nogo (RTN4) (aa14-30) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human RTN4 / Nogo
Synonyms
RTN4 | Human NogoA | My043 protein | Neurite outgrowth inhibitor | NOGOC | NSP-CL | Nogo protein | KIAA0886 | Reticulon 5 | Reticulon-4 | Reticulon-5 | Reticulon 4 | RTN4-B1 | RTN-X | RTN4-A | RTN4-B2 | RTN4-C | Nbla00271 | Neurite growth inhibitor 220 | NI220/250 | NOGO-A | Nogo-C | ASY | Foocen | Nbla10545 | NOGO | Nogo-B | NSP
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Pig
(tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A synthetic sequence corresponding to amino acids 14-30 (DSPPRPQPAFKYQFVRE) of human Nogo-B was used as the immunogen, GenBank no. AAG40878.1|AF320999_1. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Pig (100%); Bovine (94%); Mouse, Rat, Bat, Dog (88%); Opossum (82%).
Epitope
aa14-30
Specificity
This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Applications
- IHC
- IHC - Paraffin (1:100)
- Western blot (1:500 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This antibody recognizes Nogo-A (approximately 220 kD)and Nogo-B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About RTN4 / Nogo
Validation
Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-RTN4 / Nogo antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
See More About...
LSBio Ratings
IHC-plus™ RTN4 / Nogo Antibody (aa14-30) for IHC, WB/Western LS-B6516 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination. Sissy Kalayil, Sagar Bhogaraju, Florian Bonn, Donghyuk Shin, Yaobin Liu, Ninghai Gan, Jérôme Basquin, Paolo Grumati, Zhao-Qing Luo, Ivan Dikic. Nature. 2018 May;557:734-738.
The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host. Seongok Kim, Ralph R Isberg. Proceedings of the National Academy of Sciences of the United States of America. 2023 August;120:e2303942120.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
