Target
Human PSMB8 / LMP7
Synonyms
PSMB8 | D6S216 | ALDD | Low molecular mass protein 7 | LMP7 | JMP | Proteasome subunit beta 5i | Proteasome subunit beta type-8 | PSMB5i | Macropain subunit C13 | Y2 | NKJO | Protease component C13 | Proteasome component C13 | Proteasome subunit beta-5i | Proteasome-related gene 7 | RING10 | Beta5i | D6S216E | Low molecular weight protein 7 | Proteasome subunit Y2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DVSDLLHQYREANQ, from the C-Terminus of protein sequence according to NP_004150.1NP_683720.2.
Specificity
Human PSMB8. This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.01 - 0.03 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 22kDa band observed in Human Liver, Lung and Lymph Node lysates (calculated MW of 29.8kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15;165(2):768-78. PMID: 10878350. Recommended concentration: 0.01-0.03 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee