Human PLP1 / Myelin PLP

Mouse

Human, Mammal (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Purified

Unmodified

Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-Terminus peptide.

C-Terminus

Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.

• IHC
• IHC - Paraffin (10 µg/ml)
• IHC - Frozen
• ICC
• Western blot

Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4% PFA fixed cells for 5-10 minutes at RT. Use 0.1% triton in block only to allow antibody access to C-terminal epitope. Immunohistochemistry (Frozen): Fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization. Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. Positive control: Brain.

PBS, pH 7.2, 0.09% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000533 NP_000524.3