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Antibodies > PGAP1 > LS-B17024
 
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Catalog Number Size Price
LS-B17024-50 50 µl $460 
PGAP1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
PGAP1 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
PGAP1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with PGAP1 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
PGAP1 Antibody - All lanes: Anti-PGAP1 Antibody (N-Term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: mouse kidney lysate Lane 5: mouse testis lysate Lane 6: rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PGAP1 Antibody - Overlay histogram showing Hela cells stained with PGAP1 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PGAP1 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
PGAP1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
PGAP1 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
PGAP1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PGAP1 with PGAP1 Antibody (N-Term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
PGAP1 Antibody - All lanes: Anti-PGAP1 Antibody (N-Term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: mouse kidney lysate Lane 5: mouse testis lysate Lane 6: rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
PGAP1 Antibody - Overlay histogram showing Hela cells stained with PGAP1 Antibody (N-Term) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PGAP1 Antibody (N-Term), 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight®488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PGAP1 Antibody (N‑Terminus, IHC, IF, WB) LS‑B17024

IHC‑plus™ Polyclonal Rabbit anti‑Human PGAP1 Antibody (N‑Terminus, IHC, IF, WB) LS‑B17024

IHC Validated
(4.0) Rating
Submit Review LSBio Guarantee
Note: This antibody replaces LS-C757428
Antibody:
PGAP1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B17024-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PGAP1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PGAP1 antibody LS-B17024 is an unconjugated rabbit polyclonal antibody to PGAP1 (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PGAP1
Synonyms
PGAP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Ig Polyclonal
Conjugations
Unconjugated
Purification
Protein A and Peptide Affinity Chromatography
Modifications
Unmodified
Immunogen
This PGAP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 90-122 amino acids from human PGAP1.
Epitope
N-Terminus
Applications
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 105.4 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PGAP1
Q75T13

Validation

PGAP1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
PGAP1 Antibody - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ PGAP1 Antibody (N-Terminus) for IHC, IF/Immunofluorescence, WB/Western, Flow, ELISA LS-B17024 has an LSBio Rating of
(4.0) Rating
Laboratory Validation Score (4)

Publications (0)

Submit a Publication for LS-B17024

Customer Reviews (0)

Submit a review for LS-B17024

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/26/2024
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