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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3446-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human PECAM‑1 / CD31 Antibody (clone 2F7B2, IHC, IF, WB) LS‑B3446
IHC‑plus™ Monoclonal Mouse anti‑Human PECAM‑1 / CD31 Antibody (clone 2F7B2, IHC, IF, WB) LS‑B3446
Note: This antibody replaces LS-C41483
Antibody:
PECAM-1 / CD31 Mouse anti-Human Monoclonal (2F7B2) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PECAM-1 / CD31 Mouse anti-Human Monoclonal (2F7B2) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD31 antibody LS-B3446 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human PECAM-1 / CD31
Synonyms
PECAM1 | CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM-1 | GPIIA
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
2F7B2
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of human CD31 expressed in E. Coli.
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3446 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PECAM-1 / CD31
Validation
Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-CD31 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ PECAM-1 / CD31 Antibody (clone 2F7B2) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3446 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (3)
Vascular pathology in dermatomyositis and anatomic relations to myopathology. Pestronk A, Schmidt RE, Choksi R. Muscle & nerve. 2010 42:53-61. (IHC; Human)
Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis. Kerrie B Collins, Hojin Kang, Jacob Matsche, Jennifer E Klomp, Jalees Rehman, Asrar B Malik, Andrei V Karginov. The Journal of cell biology. 2020 February;219:
The Histopathological Correlate of Peri-Vascular Adipose Tissue Attenuation on Computed Tomography in Surgical Ascending Aorta Aneurysms: Is This a Measure of Tissue Inflammation?. Nicola Gaibazzi, Domenico Tuttolomondo, Francesco Nicolini, Alessandro Tafuni, Daniele Sartorio, Chiara Martini, Francesco Maestri, Alan Gallingani, Massimo De Filippo, Domenico Corradi. Diagnostics (Basel, Switzerland). 2021 Sep;11:1799.
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/25/2024
Date Requested: 12/25/2024
