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IHC‑plus™ Monoclonal Mouse anti‑Human PAX8 Antibody (clone 1F8‑3A8, IHC, IF, WB) LS‑B13466
IHC‑plus™ Monoclonal Mouse anti‑Human PAX8 Antibody (clone 1F8‑3A8, IHC, IF, WB) LS‑B13466
Note: This antibody replaces LS-C355185
Antibody:
PAX8 Mouse anti-Human Monoclonal (1F8-3A8) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Primate
Format:
Unconjugated, Unmodified
Overview
Antibody:
PAX8 Mouse anti-Human Monoclonal (1F8-3A8) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Primate
Format:
Unconjugated, Unmodified
Specifications
Description
PAX8 antibody LS-B13466 is an unconjugated mouse monoclonal antibody to PAX8 from human. It is reactive with human, mouse and primate. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PAX8
Synonyms
PAX8 | Paired box 8 | Paired domain gene 8 | Paired box gene 8 | Paired box protein Pax-8
Reactivity
Human, Mouse, Primate
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Purified internal fragment of human recombinant PAX8 expressed in E. coli.
Specificity
Western blot analysis detects a prominent ~48 kD protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Antigen retrieval with HIER and 10 mM sodium citrate is recommended for IHC using FFPE tissue samples.
Presentation
PBS, 1 mg/ml BSA, 0.05% sodium azide, 30% glycerol.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Dog, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Rabbit, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
ICC, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Species:
Human
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Human, Mouse, Rat
Range:
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/4/2025