Target
Human PAX8
Synonyms
PAX8 | Paired box 8 | Paired domain gene 8 | Paired box gene 8 | Paired box protein Pax-8
Reactivity
Human, Monkey, Mouse, Rat, Dog, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence PHNSIRSGHGGLN-C, from the N-Terminus of protein sequence according to NP_003457.1NP_039245.1NP_039246.1NP_039247.1NP_054698.1.
Specificity
Human PAX8. This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B2 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee