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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3968-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human NR1I2 / PXR Antibody (aa406‑420, IHC, WB) LS‑B3968
IHC‑plus™ Polyclonal Goat anti‑Human NR1I2 / PXR Antibody (aa406‑420, IHC, WB) LS‑B3968
Note: This antibody replaces LS-C87400
Antibody:
NR1I2 / PXR Goat anti-Human Polyclonal (aa406-420) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NR1I2 / PXR Goat anti-Human Polyclonal (aa406-420) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PXR antibody LS-B3968 is an unconjugated goat polyclonal antibody to human PXR (NR1I2) (aa406-420). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NR1I2 / PXR
Synonyms
NR1I2 | BXR | Orphan nuclear receptor PXR | Orphan nuclear receptor PAR1 | PARq | PRR | PXR | Pregnane X receptor | ONR1 | SAR | SXR
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3NP_071285.1NP_148934.1.
Epitope
aa406-420
Specificity
Human NR1I2 / PXR. This antibody is expected to recognize all reported isoforms (NP_071285.1; NP_148934.1; NP_003880.3).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 48kD band observed in lysates of cell line HepG2 (calculated MW of 49.8kD according to NP_003880.3). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NR1I2 / PXR
Validation
Anti-PXR antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PXR antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NR1I2 / PXR Antibody (aa406-420) for IHC, WB/Western LS-B3968 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
Date Requested: 11/15/2024
