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| Catalog Number | Size | Price |
|---|---|---|
| LS-B509-50 | 50 µl (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human NPC / NPC1 Antibody (C‑Terminus, IHC, IF, WB) LS‑B509
IHC‑plus™ Polyclonal Rabbit anti‑Human NPC / NPC1 Antibody (C‑Terminus, IHC, IF, WB) LS‑B509
Note: This antibody replaces LS-C2579
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
NPC1 antibody LS-B509 is an unconjugated rabbit polyclonal antibody to NPC1 (NPC) (C-Terminus) from human. It is reactive with human, mouse, hamster and other species. Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human NPC / NPC1
Synonyms
NPC1 | NPC | Niemann-Pick C1 protein | Niemann-Pick disease, type C1
Host
Rabbit
Reactivity
Human, Mouse, Hamster, Primate
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to the C-Terminus of human NPC1.
Epitope
C-Terminus
Specificity
NPC1 protein.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- Immunofluorescence (1:250)
- Western blot (1:1000 - 1:3000)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B509 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B509 was determined to be 5-10 ug/ml.
Presentation
0.025% sodium azide.
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NPC / NPC1
Validation
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-NPC1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ NPC / NPC1 Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B509 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Mouse
Applications:
Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/18/2024
Date Requested: 11/18/2024
