Human MMP9 / Gelatinase B

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Purified human gelatinase B. Cellular Localization: Peripheral cytoplasmic.

Recognizes human MMP-9 at 92kD and 86kD (non-reducing conditions) which are identified as pro (latent) and active forms of matrix metalloproteinase-9 (MMP-9; also known as 92kD and collagenase IV or gelatinase B). Reacts with the complex (~200kD) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1)1. Does not cross-react with pro and active forms of MMP-2.

• IHC
• IHC - Paraffin (5 µg/ml)
• IHC - Frozen (1:100)
• Western blot
• Immunoprecipitation

Immunohistochemistry: LS-B2371 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2371 was determined to be 5 ug/ml. Western Blot: 1 ug/ml for 2hrs at RT under non-reducing conditions. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein G. Inhibition: 2-4 ug/ml.

PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14780 NCBI: NM_004994 NP_004985.2