Human IL1R1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

IL1-31-22.2.1

Unconjugated

Protein G purified

Unmodified

The extracellular domain of the type I human IL-1 receptor.

Extracellular Domain

These antibodies react with cell surface and soluble human IL-1 receptor; Specific epitopes are unknown, but they do partially inhibit (30-50%) binding of one another to IL-1 receptor. They do not block binding of IL-1 to receptor, and they do not cross-react with receptors for human IL-2 or IL-4.

• IHC
• IHC - Paraffin (10 µg/ml)
• ELISA
• Radioimmunoassay

• Western blot

Immunohistochemistry: LS-B2859 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2859 was determined to be 10 ug/ml. ELISA: Can be used as capture reagents or second antibody at a concentration of 5 ug/ml. RIA: Concentrations of 20-30 ng/ml of these antibodies will bind radiolabeled IL-1 receptor in a radioimmunoassay format. Immunocytochemistry: IL-1 receptor can be detected on cultured cells using antibody concentrations of 10-100 ug/ml.

PBS, pH 7.4

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14778 NCBI: NM_000877 NP_000868.1