5 of 5
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5
IHC‑plus™ Monoclonal Mouse anti‑Human HSP90B1 / GP96 / GRP94 Antibody (clone 2H3, aa676‑803, IHC, IF, WB) LS‑B2929
IHC‑plus™ Monoclonal Mouse anti‑Human HSP90B1 / GP96 / GRP94 Antibody (clone 2H3, aa676‑803, IHC, IF, WB) LS‑B2929
Note: This antibody replaces LS-C93799
Antibody:
HSP90B1 / GP96 / GRP94 Mouse anti-Human Monoclonal (aa676-803) (2H3) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
HSP90B1 / GP96 / GRP94 Mouse anti-Human Monoclonal (aa676-803) (2H3) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
GRP94 antibody LS-B2929 is an unconjugated mouse monoclonal antibody to human GRP94 (HSP90B1 / GP96) (aa676-803). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSP90B1 / GP96 / GRP94
Synonyms
HSP90B1 | ECGP | Endoplasmin | gp96 homolog | gp96 | GRP-94 | GRP94 | Tumor rejection antigen 1 | TRA1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Recombinant human TRA1 (676-803aa) purified from E. coli.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (1:100 - 1:200)
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2929 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2929 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSP90B1 / GP96 / GRP94
Validation
Anti-HSP90B1 / GRP94 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSP90B1 / GRP94 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSP90B1 / GRP94 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSP90B1 / GRP94 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSP90B1 / GRP94 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSP90B1 / GRP94 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Monkey, Mouse, Bat, Dog, Hamster, Horse, Rabbit
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Horse, Chicken, Xenopus, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Chicken, Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Xenopus
Applications:
Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Reactivity:
Human
Range:
0.312-20 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 3/7/2025