Target
Human HSP90B1 / GP96 / GRP94
Synonyms
HSP90B1 | ECGP | Endoplasmin | gp96 homolog | gp96 | GRP-94 | GRP94 | Tumor rejection antigen 1 | TRA1
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide derived from the N-Terminus of the mouse and human gp96 protein. Species sequence Homology: Pig, cow, and dog: 100%.
Specificity
Recognizes mouse, rat and human gp96 proteins. Reactivity has been confirmed with mouse and rat brain homogenates by Western Blot and immunoprecipitation, and with formalin-fixed, paraffin-embedded (FFPE) human tonsil tissue by immunohistochemistry.
Applications
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation (1 - 5 µg/ml)
- ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Identifies a single band at ~96kD. Immunoprecipitation: 1-5 ug/ml. Immunohistochemistry: 0.5-1 ug/ml Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining for formalin-fixed, paraffin-embedded (FFPE) tissues.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSP90B1 / GP96 / GRP94