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| Catalog Number | Size | Price |
|---|---|---|
| LS-B517-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human HIF2A / EPAS1 Antibody (C‑Terminus, IHC) LS‑B517
IHC‑plus™ Polyclonal Rabbit anti‑Human HIF2A / EPAS1 Antibody (C‑Terminus, IHC) LS‑B517
Note: This antibody replaces LS-C2565
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
EPAS1 antibody LS-B517 is an unconjugated rabbit polyclonal antibody to EPAS1 (HIF2A) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human HIF2A / EPAS1
Synonyms
EPAS1 | BHLHe73 | ECYT4 | HIF-1-alpha-like factor | HIF2-alpha | HIF2A | EPAS-1 | HIF-2-alpha | HIF-1alpha-like factor | MOP2 | Member of PAS protein 2 | PASD2
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Sheep, Fish, Primate
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A peptide derived from the C-Terminus of mouse/human HIF-2 alpha protein.
Epitope
C-Terminus
Specificity
Specific for HIF-2 alpha/EPAS. Does not cross-react with HIF-1 alpha.
Applications
- IHC
- IHC - Paraffin (10 - 20 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B517 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B517 was determined to be 10-20 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HIF2A / EPAS1
Validation
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ HIF2A / EPAS1 Antibody (C-Terminus) for IHC LS-B517 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024
Date Requested: 11/12/2024
