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IHC‑plus™ Monoclonal Mouse anti‑Rat HCN1 Antibody (clone S70‑28, aa778‑910, IHC, IF, WB) LS‑B6436
IHC‑plus™ Monoclonal Mouse anti‑Rat HCN1 Antibody (clone S70‑28, aa778‑910, IHC, IF, WB) LS‑B6436
Note: This antibody replaces LS-C109003
Antibody:
HCN1 Mouse anti-Rat Monoclonal (aa778-910) (S70-28) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Overview
Antibody:
HCN1 Mouse anti-Rat Monoclonal (aa778-910) (S70-28) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
HCN1 antibody LS-B6436 is an unconjugated mouse monoclonal antibody to HCN1 (aa778-910) from rat. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
Target
Rat HCN1
Synonyms
HCN1 | BCNG1 | BCNG-1 | HAC2 | HAC-2
Reactivity
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
Atto 594,
APC,
FITC,
Biotin,
AP,
PerCP,
RPE,
Atto 488,
Atto 390.
Purification
Protein G purified from tissue culture supernatant
Immunogen
Fusion protein amino acids 778-910 (C-Terminus) of rat HCN1, accession number Q9JKB0.
Specificity
Detects ~100 kD protein. No cross reactivity against HCN2.
Applications
- IHC
- IHC - Paraffin (1:50)
- ICC (0.1 - 1 µg/ml)
- Immunofluorescence
- Western blot (1 - 10 µg/ml)
- Immunoprecipitation (1 - 10 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Western Blot: Use the lysate in SDS buffer but without boiling, incubating for 20 mins at 37°C instead.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
IHC analysis of HCN1 in frozen sections of mouse brain extract using HCN1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC analysis of HCN1 in frozen sections of mouse brain extract using HCN1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HCN1 antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HCN1 antibody IHC of human pancreas, islets of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50. This image was taken for the unconjugated form of this product. Other forms have not been tested.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human, Mouse
Applications:
Western blot, ELISA
Species:
Human, Mouse, Rat, Rabbit
Applications:
Western blot, ELISA
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024