Human HCN1

Rabbit

Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

A 23 aa peptide sequence corresponding to the pore region of human HCN1 corresponding to amino acids 346-368.

The pore region of HCN1 (HCNP15-P) is very conserved among various HCNs. HCN1: mouse, rat, human, rabbit=100%; HCN2: mouse, rat, human (86%); HCN3: mouse (95%), rat (90%); HCN4: rat, human (95%). The HCNP15-P sequence is also conserved in insect (Helothis virescens, 78%), Drosophila (78%) and Sea urchin (S. purpuratus, 69%) HCNs. Therefore, antibodies to HCNP15-P should react with most HCNs and related sequences. Antibody reactivity in various species is not known. The HCNP15-P control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity. This antibody may be useful in studying various HCNs and to examine the possibility of immunoblocking the pore region and impact on the functionality of HCNs proteins.

• Western blot (1 - 10 µg/ml)
• ELISA (1:10000 - 1:100000)

Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.

PBS, pH 7.4, 0.1% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O60741 NCBI: NM_021072 AAC39759.1