Human GRN / Granulin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide derived from the C-Terminus of human PCDGF (PC cell-derived growth factor, GP88, acrogranin, granulin precursor, epithelin precursor, or progranulin)

C-Terminus

Recognizes human PCDGF protein at ~64-88kD.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 3 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B3158 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3158 was determined to be 5 ug/ml. Positive control: Human MDA-MB-231 breast adenocarcinoma, PC-3 prostate adenocarcinoma (bone metastatic site), HeLa cervical carcinoma, and HT1080 fibrosarcoma lysates.

PBS, pH 7.4, 0.09% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P28799 NCBI: NM_002087 NP_002078.1