Rat GRB2

Mouse

Rat, Human, Mouse, Bovine, Hamster (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Recombinant rat GRB2/Ash.

Recognizes the N-terminal SH3 region of rat Grb2 at 23-25kD.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (0.5 - 2 µg/ml)

Immunohistochemistry: LS-B3068 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3068 was determined to be 5 ug/ml. Western Blot: 0.5-2 mg/ml. Detects GRB2 (23-25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells and bovine brain extract.

PBS, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P62993 NCBI: NM_002086 NP_002077.1