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| Catalog Number | Size | Price |
|---|---|---|
| LS-B83-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Pig GAPDH Antibody (clone 1D4, IHC, IF, WB) LS‑B83
IHC‑plus™ Monoclonal Mouse anti‑Pig GAPDH Antibody (clone 1D4, IHC, IF, WB) LS‑B83
Note: This antibody replaces LS-C2675
Antibody:
GAPDH Mouse anti-Pig Monoclonal (1D4) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Pig, Human, Mouse, Rat, Bovine, Hamster, Opossum, Rabbit, Sheep, Chicken, Avian, Primate
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GAPDH Mouse anti-Pig Monoclonal (1D4) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Pig, Human, Mouse, Rat, Bovine, Hamster, Opossum, Rabbit, Sheep, Chicken, Avian, Primate
Format:
Unconjugated, Unmodified
Specifications
Description
GAPDH antibody LS-B83 is an unconjugated mouse monoclonal antibody to GAPDH from pig. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Target
Pig GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Mouse
Reactivity
Pig, Human, Mouse, Rat, Bovine, Hamster, Opossum, Rabbit, Sheep, Chicken, Avian, Primate
(tested or 100% immunogen sequence identity)
Clonality
IgM,k
Monoclonal
Clone
1D4
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
The immunogen used to raise this particular antibody was extensively purified pig GAPDH.
Specificity
GAPDH (1D4).
Applications
- IHC
- IHC - Paraffin (1.25 - 2.5 µg/ml)
- Immunofluorescence (1:100)
- Western blot (1:1000)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B83 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B83 was determined to be 1.25-2.5 ug/ml.
Presentation
50% PBS, 50% Glycerol, 5mm sodium azide.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GAPDH
Validation
Anti-GAPDH antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GAPDH antibody IHC of human brain cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ GAPDH Antibody (clone 1D4) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B83 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Bovine, Cat, Dog, Goat, Pig, Rabbit, Fish
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Species:
Human, Mouse, Rat, Drosophila
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Pig, Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
