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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2536-50 | 50 µg (1 mg/ml) | $440 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human GAPDH Antibody (aa73‑87, IHC, WB) LS‑B2536
IHC‑plus™ Polyclonal Rabbit anti‑Human GAPDH Antibody (aa73‑87, IHC, WB) LS‑B2536
Note: This antibody replaces LS-C19379, LS-C66761
Antibody:
GAPDH Rabbit anti-Human Polyclonal (aa73-87) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GAPDH Rabbit anti-Human Polyclonal (aa73-87) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
GAPDH antibody LS-B2536 is an unconjugated rabbit polyclonal antibody to GAPDH (aa73-87) from human. It is reactive with human and monkey. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Rabbit
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Predicted
Pig (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset (100%); Pig (93%); Mouse, Rat, Cat, Chicken, Armadillo, Xenopus (87%).
Epitope
aa73-87
Specificity
Amino acids 73-87 (PITIFQERDPSKIKW) of glyceraldehyde 3-phosphate dehydrogenase protein was used as the immunogen.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2536 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2536 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GAPDH
Validation
Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-GAPDH antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ GAPDH Antibody (aa73-87) for IHC, WB/Western LS-B2536 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Mouse, Rat, Bovine, Cat, Dog, Goat, Pig, Rabbit, Fish
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Species:
Human, Mouse, Rat, Drosophila
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Pig, Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
