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| Catalog Number | Size | Price |
|---|---|---|
| LS-B12855-250 | 250 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human GAPDH Antibody (clone 1D4, IHC, IF, WB) LS‑B12855
IHC‑plus™ Monoclonal Mouse anti‑Human GAPDH Antibody (clone 1D4, IHC, IF, WB) LS‑B12855
Note: This antibody replaces LS-C204531
Antibody:
GAPDH Mouse anti-Human Monoclonal (1D4) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GAPDH Mouse anti-Human Monoclonal (1D4) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
GAPDH antibody LS-B12855 is an unconjugated mouse monoclonal antibody to GAPDH from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Pig
(tested or 100% immunogen sequence identity)
Clonality
IgM,k
Monoclonal
Clone
1D4
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
The immunogen used to raise this antibody was extensively purified pig GAPDH.
Specificity
Clone 1D4 recognizes GAPDH specifically both in western blots and in immunocytochemical experiments. On blots clone 1D4 reveals a prominent ~38kDa band, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion. clone 1D4 is known to react with GAPDH from human, cow, pig, mouse, rat and other mammals, and also recognizes avian GAPDH. Since GAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.
Applications
- IHC
- IHC - Paraffin (1:50)
- ICC (1:100)
- Immunofluorescence (1:100)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The antibody solution can be used at dilutions of at least 1:100 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000 or lower. It is very useful as a loading control for quantitative western blotting.
Presentation
Tissue Culture Supernatant, 10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GAPDH
Validation
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ GAPDH Antibody (clone 1D4) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B12855 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
Date Requested: 11/23/2024
