Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-B9169-50	50 µg (0.5 mg/ml)	$485

GAPDH Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

GAPDH Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

GAPDH Antibody - GAPDH (C Terminus) Loading Control Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

GAPDH Antibody - GAPDH antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Human Liver (A) and Tonsil (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of lysates of cell line HEK293 (A) and HeLa (B) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GAPDH Antibody - GAPDH antibody (0.5µg/ml) staining of Mouse Liver (A) and Rat Brain (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GAPDH Antibody - Staining (0.01 ug/ml) of 293 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

GAPDH Antibody - GAPDH antibody (0.01µg/ml) staining of cell lines HEK293 (A) and HeLa (B) and Human Liver (C) and Tonsil (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

GAPDH Antibody - GAPDH antibody (0.01µg/ml) staining of Mouse Spleen (A), (0.1ug/ml) Rat Brain (B) and (0.5ug/ml) Rat Spleen (C) (35µg protein in RIPA buffer). Detected by chemiluminescence.

1 of 10

2 of 10

3 of 10

4 of 10

5 of 10

6 of 10

7 of 10

8 of 10

9 of 10

10 of 10

IHC‑plus™ Polyclonal Goat anti‑Human GAPDH Antibody (aa279‑290, IHC, IF, WB) LS‑B9169

Name: IHC-plus™ GAPDH Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B9169-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

Submit Review LSBio Guarantee

Note: This antibody replaces LS-C20160

Antibody:

GAPDH Goat anti-Human Polyclonal (aa279-290) Antibody

Application:

IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey

Format:

Unconjugated, Unmodified

$485

LS-B9169-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

GAPDH Goat anti-Human Polyclonal (aa279-290) Antibody

Application:

IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human, Monkey

Format:

Unconjugated, Unmodified

Specifications

Description

GAPDH antibody LS-B9169 is an unconjugated goat polyclonal antibody to GAPDH (aa279-290) from human. It is reactive with human and monkey. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human GAPDH

Synonyms

See All GAPDH Antibodies

Host

Goat

Reactivity

Human, Monkey (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-HQVVSSDFNSDT, from the C-Terminus of protein sequence according to NP_002037.2.

Epitope

aa279-290

Specificity

Human GAPDH. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Applications

IHC - Paraffin (1.25 - 2.5 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (0.01 - 0.5 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About GAPDH

Uniprot: P04406 NCBI: NM_002046 NP_002037.2