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| Catalog Number | Size | Price |
|---|---|---|
| LS-A4278-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human FZD5 / Frizzled 5 Antibody (N‑Terminus, IHC) LS‑A4278
IHC‑plus™ Polyclonal Rabbit anti‑Human FZD5 / Frizzled 5 Antibody (N‑Terminus, IHC) LS‑A4278
Antibody:
FZD5 / Frizzled 5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Overview
Antibody:
FZD5 / Frizzled 5 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
Frizzled 5 antibody LS-A4278 is an unconjugated rabbit polyclonal antibody to Frizzled 5 (FZD5) (N-Terminus) from human. It is reactive with human, bovine, dog and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human FZD5 / Frizzled 5
Synonyms
FZD5 | C2orf31 | DKFZP434E2135 | Frizzled family receptor 5 | Fz5 | Fzd-5 | FzE5 | Frizzled 5 | Frizzled-5 | Wnt receptor | Fz-5 | HFZ5
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Dog, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 14 amino acid peptide from N-Terminus extracellular domain of human FZD5 / Frizzled 5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bovine, Elephant, Rabbit, Pig (100%); Mouse, Rat, Hamster (93%); Opossum (86%).
Epitope
N-Terminus
Specificity
Human FZD5 / Frizzled 5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (7 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FZD5 / Frizzled 5
Validation
Anti-FZD5 / Frizzled 5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FZD5 / Frizzled 5 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ FZD5 / Frizzled 5 Antibody (N-Terminus) for IHC, ELISA LS-A4278 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Hamster
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
Western blot, ELISA
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/19/2024
Date Requested: 11/19/2024
