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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2559-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human F2 / Prothrombin / Thrombin Antibody (IHC, WB) LS‑B2559
IHC‑plus™ Monoclonal Mouse anti‑Human F2 / Prothrombin / Thrombin Antibody (IHC, WB) LS‑B2559
Note: This antibody replaces LS-C38625
Antibody:
F2 / Prothrombin / Thrombin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
F2 / Prothrombin / Thrombin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Thrombin antibody LS-B2559 is an unconjugated mouse monoclonal antibody to human Thrombin (F2 / Prothrombin). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human F2 / Prothrombin / Thrombin
Synonyms
F2 | Prothrombin B-chain | Coagulation factor II | FACTOR II | Prothrombin | PT | RPRGL2 | Serine protease | THPH1 | Thrombin
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Human Prothrombin.
Specificity
Recognizes human Prothrombin, prethrombin-1, fragment 2, meizothrombin. Inhibits clotting and prothrombin activation. Epitope: Located in the fragment 2 region (aa156-271)
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (10 µg/ml)
- ELISA (5 - 10 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2559 was determined to be 5 ug/ml.
Presentation
Supplied as a liquid in 50% glycerol, ddH2O. Sourced from ascites.
Storage
Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About F2 / Prothrombin / Thrombin
Validation
Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-F2 / Thrombin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ F2 / Prothrombin / Thrombin Antibody for IHC, WB/Western, ELISA LS-B2559 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Bovine
Applications:
ELISA, Immunoelectrophoresis
Species:
Human
Applications:
ELISA, Immunoelectrophoresis
Species:
Human
Applications:
Western blot, ELISA, Inhibition
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Bovine
Range:
7.8-500 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/25/2024
Date Requested: 11/25/2024
