Target
Human F12 / Factor XII
Synonyms
F12 | Beta-factor XIIa part 2 | Beta-factor XIIa part 1 | Coagulation factor XII | F12 deficiency | Factor XII | Factor XII deficiency | HAE3 | HAEX | Hageman factor | HAF
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Immunogen
Activated human Factor XII (F XIIa) purified from plasma.
Specificity
Specific for Factor XII as demonstrated by immunodiffusion. A single precipitin arc was obtained with normal plasma and no reaction was observed with F. XII-deficient plasma.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunoprecipitation
- ELISA
- Immunoelectrophoresis
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Usage
Immunohistochemistry: LS-B3044 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3044 was determined to be 5 ug/ml.
Presentation
10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee