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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5401-50 | 50 µg (1 mg/ml) | $460 |
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2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human EN1 / Engrailed Antibody (clone 1F5, IHC, WB) LS‑B5401
IHC‑plus™ Monoclonal Mouse anti‑Human EN1 / Engrailed Antibody (clone 1F5, IHC, WB) LS‑B5401
Note: This antibody replaces LS-C133170
Antibody:
EN1 / Engrailed Mouse anti-Human Monoclonal (1F5) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
EN1 / Engrailed Mouse anti-Human Monoclonal (1F5) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
Engrailed antibody LS-B5401 is an unconjugated mouse monoclonal antibody to Engrailed (EN1) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human EN1 / Engrailed
Synonyms
EN1 | Engrailed 1 | Engrailed homolog 1 | Homeobox protein en-1 | Homeobox protein engrailed-1 | Hu-En-1 | Engrailed homeobox 1
Host
Mouse
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
1F5
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
EN1 (NP_001417, 266 aa ~ 392 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Engrailed Homolog 1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot performed with the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-EN1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-EN1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ EN1 / Engrailed Antibody (clone 1F5) for IHC, WB/Western, ELISA LS-B5401 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/11/2025
Date Requested: 2/11/2025
