Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B9070-100 100 µl (0.45 mg/ml) $460 
EN1 / Engrailed Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
EN1 / Engrailed Antibody - Fluorescent image of U251 cell stained with EN1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EN1 immunoreactivity is localized to Nucleus significantly.
EN1 / Engrailed Antibody - Western blot of lysates from NCI-H460,293,SH-SY5Y cell line (from left to right),using EN1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
EN1 / Engrailed Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
EN1 / Engrailed Antibody - Fluorescent image of U251 cell stained with EN1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EN1 immunoreactivity is localized to Nucleus significantly.
EN1 / Engrailed Antibody - Western blot of lysates from NCI-H460,293,SH-SY5Y cell line (from left to right),using EN1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
1 of 3
2 of 3
3 of 3

IHC‑plus™ Polyclonal Rabbit anti‑Human EN1 / Engrailed Antibody (aa1‑30, IHC, IF, WB) LS‑B9070

IHC‑plus™ Polyclonal Rabbit anti‑Human EN1 / Engrailed Antibody (aa1‑30, IHC, IF, WB) LS‑B9070

Note: This antibody replaces LS-C99956
Antibody:
EN1 / Engrailed Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9070-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
EN1 / Engrailed Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Engrailed antibody LS-B9070 is an unconjugated rabbit polyclonal antibody to human Engrailed (EN1) (aa1-30). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human EN1 / Engrailed
Synonyms
EN1 | Engrailed 1 | Engrailed homolog 1 | Homeobox protein en-1 | Homeobox protein engrailed-1 | Hu-En-1 | Engrailed homeobox 1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa1-30
Specificity
This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS with 0.09% (w/v) sodium azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 2 weeks. For long term storage aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EN1 / Engrailed
Q05925 Q05925

Validation

EN1 / Engrailed Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ EN1 / Engrailed Antibody (aa1-30) for IHC, IF/Immunofluorescence, WB/Western LS-B9070 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal a-motoneurons. Mélanie Lebœuf , Stephanie E Vargas-Abonce , Eugénie Pezé-Hedsieck, Edmond Dupont, Lucia Jimenez-Alonso, Kenneth L Moya, Alain Prochiantz. EMBO reports. 2023 August;24:e56525.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/11/2025