Human EEF2 / Elongation Factor 2

Rabbit

Human, Monkey, Mouse, Rat, Hamster, Chicken (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr56 of human eEF2.

pThr56

Detects endogenous levels of eEF2 only when phosphorylated at threonine 56. It does not recognize eEF2 phosphorylated at other sites.

• IHC
• IHC - Paraffin (1:100)
• Western blot (1:1000)
• ELISA

Immunohistochemistry: LS-B2258 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2258 was determined to be 1:100. Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4uC with gentle shaking, overnight.

10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13639 NCBI: NM_001961 NP_001952.1