Target
Human EEF2 / Elongation Factor 2
Synonyms
EEF2 | EEF-2 | EF-2 | Elongation factor 2 | Polypeptidyl-tRNA translocase | EF2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide (KLH coupled) corresponding to residues mapping at the N-Terminus of human eEF2.
Specificity
Detects endogenous levels of total eEF2 independent of phosphorylation (Mr 100kD). Species cross-reactivity: Human, mouse and rat. Species sequence homology: chicken and hamster 100%.
Applications
- ICC
- Immunofluorescence
- Western blot (1:1000)
- ELISA
Usage
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunocytochemistry (for IF): 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EEF2 / Elongation Factor 2