IHC‑plus™ Polyclonal Rabbit anti‑Human DLK1 / Pref‑1 Antibody (Extracellular Domain, IHC, WB) LS‑B13733
IHC‑plus™ Polyclonal Rabbit anti‑Human DLK1 / Pref‑1 Antibody (Extracellular Domain, IHC, WB) LS‑B13733
Note: This antibody replaces LS-C345027
Antibody:
DLK1 / Pref-1 Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
DLK1 / Pref-1 Rabbit anti-Human Polyclonal (Extracellular Domain) Antibody
Application:
IHC, IHC-P, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
Pref-1 antibody LS-B13733 is an unconjugated rabbit polyclonal antibody to human Pref-1 (DLK1) (Extracellular Domain). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human DLK1 / Pref-1
Synonyms
DLK1 | Delta-like protein dlk | DLK-1 | Delta1 | FA1 | Dlk | Preadipocyte factor 1 | Pref-1 | Protein delta homolog 1 | PG2 | ZOG | Fetal antigen 1 | PREF1 | Secredeltin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Recombinant human DLK1 (extracellular domain).
Epitope
Extracellular Domain
Specificity
Recognizes human DLK1. Detects a band of ~42kDa by Western blot. Weakly cross-reacts with mouse Dlk1.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:2000 - 1:10000)
- ELISA (1:2000 - 1:10000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
ELISA - Direct and indirect: 1:2000-1:10000. Western Blot - 1:2000-1:10000 using ECL. suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Store at -20°C for up to 6 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Mouse
Applications:
Flow Cytometry
Species:
Human
Applications:
Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Mouse
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 8/31/2024