Human CTNNB1 / Beta Catenin

Rabbit

Human, Mouse, Rat, Chicken, Xenopus (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

A 15 AA synthetic peptide derived from the C-Terminus of human and mouse b-catenin protein.

C-Terminus

Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot
• Immunoprecipitation
• ELISA (0.1 - 1 µg/ml)

Immunohistochemistry: LS-B2354 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2354 was determined to be 2.5 ug/ml. Western Blot after IP: 1-2 ug/ml. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 ug. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates.

PBS, pH 7.4, 0.1% Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P35222 NCBI: NM_001904 NP_001895.1