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| Catalog Number | Size | Price |
|---|---|---|
| LS-B932-100 | 100 µl | $485 |
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Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (aa192‑241, IHC, WB) LS‑B932
Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (aa192‑241, IHC, WB) LS‑B932
Note: This antibody replaces LS-C29817
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
CHK1 antibody LS-B932 is an unconjugated rabbit polyclonal antibody to human CHK1 (CHEK1) (aa192-241). Validated for IHC and WB.
Target
Human CHEK1 / CHK1
Synonyms
CHEK1 | CHK1 checkpoint homolog | Cell cycle checkpoint kinase | Checkpoint kinase 1 | CHK1 | Chk1-S | Protein kinase chk1 | Checkpoint kinase-1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa192-241 of human CHEK1 (O14757, NP_001265). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Zebra finch (100%); Hamster, Bovine, Pig, Opossum, Guinea pig, Chicken, Platypus (92%); Stickleback (85%).
Epitope
aa192-241
Specificity
Human CHEK1 / CHK1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B932 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B932 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CHEK1 / CHK1
Publications (0)
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Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Dot Blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
![CHEK1 / CHK1 Antibody - Immunofluorescence of monoclonal antibody to CHEK1 on HeLa cell . [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/chek1-chk1-antibody-clone-2g3-ls-c196906/144310_498563.jpg)
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/17/2024
Date Requested: 12/17/2024
