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Catalog Number	Size	Price
LS-C358948-30	30 µl (1 mg/ml)	$263
LS-C358948-100	100 µl (1 mg/ml)	$315
LS-C358948-200	200 µl (1 mg/ml)	$379

CHEK1 / CHK1 Antibody - Immunohistochemical analysis of CHK1 (pS345) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

CHEK1 / CHK1 Antibody - Immunofluorescent analysis of CHK1 (pS345) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CHEK1 / CHK1 Antibody - Western blot analysis of CHK1 (pS345) expression in HeLa (A); Jurkat (B); HL60 (C); mouse brain (D) whole cell lysates.

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Polyclonal Rabbit anti‑Human CHEK1 / CHK1 Antibody (phospho‑Ser345, IHC, IF, WB) LS‑C358948

Name: Anti-CHEK1 / CHK1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C358948-30
Price: 263.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer345) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$263

LS-C358948-30

$315

LS-C358948-100

$379

LS-C358948-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CHEK1 / CHK1 Rabbit anti-Human Polyclonal (pSer345) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

CHK1 antibody LS-C358948 is an unconjugated rabbit polyclonal antibody to CHK1 (CHEK1) (pSer345) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human CHEK1 / CHK1

Synonyms

See All CHEK1 / CHK1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human CHK1.

Epitope

pSer345

Specificity

Recognizes endogenous levels of CHK1 (pS345) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CHEK1 / CHK1

Uniprot: O14757 NCBI: NM_001274 NP_001265.2

LSBio Ratings

CHEK1 / CHK1 Antibody (phospho-Ser345) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C358948 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

JAK Inhibition Prevents DNA Damage and Apoptosis in Testicular Ischemia-Reperfusion Injury via Modulation of the ATM/ATR/Chk Pathway. Farah Khashab, Farah Al-Saleh, Nora Al-Kandari, Fatemah Fadel, May Al-Maghrebi. International journal of molecular sciences. 2021 December;22:

Full Text Article PubMed: 34948185 PMC: PMC8706947

Submit a Publication for LS-C358948