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Catalog Number Size Price
LS-B11911-50 50 µl (2.37 mg/ml) $460 
CD86 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD86 Antibody - Immunofluorescence analysis of MCF-7 cells using CD86 antibody. Blue: DAPI for nuclear staining.
CD86 Antibody - Western blot analysis of extracts of THP-1 cells, using CD86 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
CD86 Antibody - Western blot analysis of extracts of various cell lines.
CD86 Antibody - Western blot analysis of extracts of various cell lines, using CD86 antibody.
CD86 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
CD86 Antibody - Immunofluorescence analysis of MCF-7 cells using CD86 antibody. Blue: DAPI for nuclear staining.
CD86 Antibody - Western blot analysis of extracts of THP-1 cells, using CD86 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
CD86 Antibody - Western blot analysis of extracts of various cell lines.
CD86 Antibody - Western blot analysis of extracts of various cell lines, using CD86 antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CD86 Antibody (IHC, IF, WB) LS‑B11911

IHC‑plus™ Polyclonal Rabbit anti‑Human CD86 Antibody (IHC, IF, WB) LS‑B11911

Note: This antibody replaces LS-C331350
Antibody:
CD86 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11911-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD86 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD86 antibody LS-B11911 is an unconjugated rabbit polyclonal antibody to human CD86. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD86
Synonyms
CD86 | Activation B7-2 antigen | B7-2 | B7.2 | B70 | CD86 molecule | CTLA-4 counter-receptor B7.2 | CD86 antigen | FUN-1 | LAB72 | BU63 | CD28LG2 | Ctla-4 counter-receptors b7.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-247 of human CD86 (NP_787058.4). AYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGIMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP
Specificity
Human CD86
Applications
  • IHC - Paraffin (1:100)
  • ICC (1:50 - 1:100)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 12kDa/24kDa/28kDa/31kDa/37kDa, while the observed MW by Western blot was 80kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD86
P42081 NM_006889 NP_008820.3

Validation

CD86 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ CD86 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B11911 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/25/2024