Human CCR10 / GPR2

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein G purified

Unmodified

A peptide corresponding to amino acids 350-362 of human CCR10. In this region, mouse and human amino acid sequences are 100% identical.

aa350-362

In A375, a ~50 kD band is observed. Is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2% formaldehyde and permeabilized with 0.1% saponin.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 2 µg/ml)
• Flow Cytometry

Immunohistochemistry: LS-B2023 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2023 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide, 0.2% Gelatin

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P46092 NCBI: NM_016602 NP_057686.2