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Catalog Number Size Price
LS-B2325-50 50 µg (0.5 mg/ml) $485 
CCKBR / Cckb Antibody - Anti-CCKBR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CCKBR / Cckb Antibody - CCKBR Antibody (1µg/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
CCKBR / Cckb Antibody - Anti-CCKBR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
CCKBR / Cckb Antibody - CCKBR Antibody (1µg/ml) staining of Mouse Pancreas (A) and Rat Stomach (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
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IHC‑plus™ Polyclonal Goat anti‑Human CCKBR / Cckb Antibody (aa251‑266, IHC, WB) LS‑B2325

IHC‑plus™ Polyclonal Goat anti‑Human CCKBR / Cckb Antibody (aa251‑266, IHC, WB) LS‑B2325

Note: This antibody replaces LS-C54625, LS-C79258, LS-B2855
Antibody:
CCKBR / Cckb Goat anti-Human Polyclonal (aa251-266) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B2325-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CCKBR / Cckb Goat anti-Human Polyclonal (aa251-266) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cckb antibody LS-B2325 is an unconjugated goat polyclonal antibody to human Cckb (CCKBR) (aa251-266). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human CCKBR / Cckb
Synonyms
CCKBR | CCKB | Cholecystokinin-2 receptor | CCK-BR | CCK2 receptor | CCK2-R | CCKB-R | Cholecystokinin-B | GASR | CCK-B | CCK-B receptor | CCK2R | Cckb receptor | CCKRB | Cholecystokinin B receptor | Gastrin receptor
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1.
Epitope
aa251-266
Specificity
Human CCKBR.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2325 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2325 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCKBR / Cckb
P32239 NM_176875 NP_795344.1

Validation

CCKBR / Cckb Antibody - Anti-CCKBR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CCKBR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CCKBR antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ CCKBR / Cckb Antibody (aa251-266) for IHC, WB/Western LS-B2325 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line. El-Kouhen K, Morisset J. Pancreas. 2010 39:127-34. (IF; Rat)

Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells. El-Kouhen K, Morisset J. International journal of endocrinology. 2009 2009:875167.

CCK1 and CCK2 receptors are expressed on pancreatic stellate cells and induce collagen production. Berna MJ, Seiz O, Nast JF, Benten D, Blker M, Koch J, Lohse AW, Pace A. The Journal of biological chemistry. 2010 285:38905-14.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/22/2024