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IHC‑plus™ Monoclonal Mouse anti‑Human CBFA1 / RUNX2 Antibody (clone 3F5, IHC, IF, WB) LS‑B4296
IHC‑plus™ Monoclonal Mouse anti‑Human CBFA1 / RUNX2 Antibody (clone 3F5, IHC, IF, WB) LS‑B4296
Note: This antibody replaces LS-C104878
Antibody:
CBFA1 / RUNX2 Mouse anti-Human Monoclonal (3F5) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
CBFA1 / RUNX2 Mouse anti-Human Monoclonal (3F5) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
RUNX2 antibody LS-B4296 is an unconjugated mouse monoclonal antibody to RUNX2 (CBFA1) from human. It is reactive with human and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CBFA1 / RUNX2
Synonyms
RUNX2 | AML3 | CCD | CCD1 | OSF2 | PEBP2A1 | PEBP2A2 | PEA2-alpha A | PEBP2-alpha A | OSF-2 | PEBP2A | PEBP2aA1 | CBF-alpha-1 | CBFA1 | Oncogene AML-3 | PEA2aA | PEBP2aA
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Immunogen
RUNX2 (NP_004339, 251 aa ~ 351 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (10 µg/ml)
- Western blot (1:500 - 1:1000)
- ELISA
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot (Cell lysate) - positive control PC-12. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Validation
Anti-RUNX2 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RUNX2 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RUNX2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
LSBio Ratings
Laboratory Validation Score (4)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Rat
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot
Species:
Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Horse, Pig, Chicken
Applications:
Western blot
Species:
Human
Applications:
Western blot
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/6/2025