Human CASP1 / Caspase 1

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

Rat (at least 90% immunogen sequence identity)

IgG1,k Monoclonal

14F468

Unconjugated

Protein G purified

Unmodified

Two synthetic peptides from the human caspase-1 protein (aa 371-390 and aa 31-45)

aa371-390

will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.

• IHC
• IHC - Paraffin (20 µg/ml)
• ICC
• Western blot (0.5 - 2 µg/ml)

Immunohistochemistry: LS-B112 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B112 was determined to be 20 ug/ml.

PBS, 0.02% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P29466 NCBI: NM_001223 NP_001214.1