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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3886-50 | 50 µl | $485 |
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IHC‑plus™ Monoclonal Mouse anti‑Human CANX / Calnexin Antibody (clone 3H4A7, IHC, IF, WB) LS‑B3886
IHC‑plus™ Monoclonal Mouse anti‑Human CANX / Calnexin Antibody (clone 3H4A7, IHC, IF, WB) LS‑B3886
Note: This antibody replaces LS-C108774
Antibody:
CANX / Calnexin Mouse anti-Human Monoclonal (3H4A7) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CANX / Calnexin Mouse anti-Human Monoclonal (3H4A7) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Calnexin antibody LS-B3886 is an unconjugated mouse monoclonal antibody to human Calnexin (CANX). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CANX / Calnexin
Synonyms
CANX | CNX | Calnexin | IP90 | p90
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
3H4A7
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH.
Specificity
Human
Applications
- IHC
- IHC - Paraffin (1:200 - 1:400)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3886 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3886 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CANX / Calnexin
Validation
Anti-Calnexin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calnexin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ CANX / Calnexin Antibody (clone 3H4A7) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3886 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Applications:
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
