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Catalog Number	Size	Price
LS-B16042-50	50 µg (0.5 mg/ml)	$460

c-Kit / CD117 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

c-Kit / CD117 Antibody - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

c-Kit / CD117 Antibody - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

c-Kit / CD117 Antibody - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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IHC‑plus™ Polyclonal Goat anti‑Human c‑Kit / CD117 Antibody (IHC, IF) LS‑B16042

Name: IHC-plus™ c-Kit / CD117 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B16042-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C758304

Antibody:

c-Kit / CD117 Goat anti-Human Polyclonal Antibody

Application:

IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$460

LS-B16042-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

c-Kit / CD117 Goat anti-Human Polyclonal Antibody

Application:

IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

CD117 antibody LS-B16042 is an unconjugated goat polyclonal antibody to CD117 (c-Kit) from human. It is reactive with human and mouse. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.

Target

Human c-Kit / CD117

Synonyms

See All c-Kit / CD117 Antibodies

Host

Goat

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-HHGDFNYERQAT, from the internal region of the protein sequence according to NP_000213.1NP_001087241.1.

Specificity

This antibody is expected to recognise both reported isoforms (NP_000213.1; NP_001087241.1)

Applications

IHC - Paraffin (5 µg/ml)
Immunofluorescence (10 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Peptide ELISA: antibody detection limit dilution 1:16000. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 and in the membranes of NIH3T3 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About c-Kit / CD117

Uniprot: P10721 NCBI: NM_000222 NP_000213.1