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| Catalog Number | Size | Price |
|---|---|---|
| LS-B4289-50 | 50 µg | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone 2G5, IHC, WB) LS‑B4289
IHC‑plus™ Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone 2G5, IHC, WB) LS‑B4289
Note: This antibody replaces LS-C104869
Antibody:
BUB1B / BubR1 Mouse anti-Human Monoclonal (2G5) Antibody
Application:
IHC, IHC-P, WB, ELISA, PLA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
BUB1B / BubR1 Mouse anti-Human Monoclonal (2G5) Antibody
Application:
IHC, IHC-P, WB, ELISA, PLA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
BubR1 antibody LS-B4289 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for ELISA, IHC, PLA, RNAi and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BUB1B / BubR1
Synonyms
BUB1B | Bub1A | BUBR1 | HBUBR1 | Protein SSK1 | SSK1 | BUB1beta | MAD3L
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG,k
Monoclonal
Clone
2G5
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
BUB1B (AAH18739, 1 aa ~ 130 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
- ELISA
- Proximity Ligation Assay
- RNA interference
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated). Proximity Ligation Assay (Cell).
Presentation
PBS, pH 7.4
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BUB1B / BubR1
Validation
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ BUB1B / BubR1 Antibody (clone 2G5) for IHC, WB/Western, ELISA LS-B4289 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
![BUB1B / BubR1 Antibody - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with BUBR1 [8G1] antibody. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/bub1b-bubr1-antibody-aa1-350-clone-8g1-ls-c2771/3447_423118.jpg)
Species:
Human, Mouse
Applications:
Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human, Monkey, Rat, Dog
Applications:
Immunofluorescence, Western blot, Flow Cytometry
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
