Human B2M / Beta 2 Microglobulin

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Bovine, Dog, Rabbit, Chicken

IgG2a Monoclonal

B2M-01

Unconjugated

Purified

Concentrated

Purified human beta2-microglobulin

Reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. b2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes,thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

• IHC
• IHC - Paraffin (1:50)
• Western blot (2 - 4 µg/ml)
• Immunoprecipitation
• Flow Cytometry
• ELISA
• Radioimmunoassay

For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.

PBS containing 15 mM in sodium azide, pH 7.4

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P61769 NCBI: NM_004048 NP_004039.1