Target
Human APOC3 / Apolipoprotein C III
Synonyms
APOC3 | Apo-CIII | Apolipoprotein C3 | APOCIII | HALP2 | ApoC-III | Apolipoprotein C-III
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic full-length Apolipoprotein Type C-III
Specificity
Recognizes human apolipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apolipoprotein. Non-specific cross-reactivity of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:500 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:10000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B3581 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3581 was determined to be 2.5 ug/ml. Western Blot: 1:500-1:5000. ELISA: 1:5000-1:10000. Immunoprecipitation: 1:100.
Presentation
0.125 M Sodium Borate, pH 8.0, 0.075 M NaCl, 0.005 M EDTA, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APOC3 / Apolipoprotein C III