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Catalog Number	Size	Price
LS-B15324-50	50 µl	$460

APG5 / ATG5 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.

APG5 / ATG5 Antibody - Immunohistochemistry of paraffin-embedded human prostate, with a concentration of 1:100.

APG5 / ATG5 Antibody - Immunofluorescence analysis of HeLa cells using ATG5 antibodyat dilution of 1:100. Blue: DAPI for nuclear staining.

APG5 / ATG5 Antibody - Western blot analysis of extracts of various cell lines, using ATG5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

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IHC‑plus™ Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (IHC, IF, WB) LS‑B15324

Name: IHC-plus™ APG5 / ATG5 Polyclonal Antibody Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B15324-50
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C330829

Antibody:

APG5 / ATG5 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$460

LS-B15324-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

APG5 / ATG5 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

ATG5 antibody LS-B15324 is an unconjugated rabbit polyclonal antibody to ATG5 (APG5) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human APG5 / ATG5

Synonyms

See All APG5 / ATG5 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1). MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD

Specificity

Human APG5 / ATG5

Applications

IHC
IHC - Paraffin (1:200)
Immunofluorescence
Western blot (1:500 - 1:2000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

The predicted MW is 22kDa/32kDa, while the observed MW by Western blot was 58kDa.

Presentation

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About APG5 / ATG5