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Catalog Number Size Price
LS-B11793-200 200 µl $440 
APG5 / ATG5 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
APG5 / ATG5 Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
APG5 / ATG5 Antibody - Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG5 / ATG5 Antibody - The anti-APG5L antibody is used in Western blot to detect APG5L in mouse brain tissue lysate.
APG5 / ATG5 Antibody - Western blot of APG5L (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.
APG5 / ATG5 Antibody - Western blot of APG5L Antibody (D3) (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
APG5 / ATG5 Antibody - APG5L Antibody (D3) western blot of HeLa cell line lysates (35 ug/lane). The APG5L antibody detected the APG5L protein (arrow).
APG5 / ATG5 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
APG5 / ATG5 Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
APG5 / ATG5 Antibody - Fluorescent image of U251 cells stained with ATG5 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG5 / ATG5 Antibody - The anti-APG5L antibody is used in Western blot to detect APG5L in mouse brain tissue lysate.
APG5 / ATG5 Antibody - Western blot of APG5L (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.
APG5 / ATG5 Antibody - Western blot of APG5L Antibody (D3) (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
APG5 / ATG5 Antibody - APG5L Antibody (D3) western blot of HeLa cell line lysates (35 ug/lane). The APG5L antibody detected the APG5L protein (arrow).
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IHC‑plus™ Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (aa1‑30, IHC, IF, WB) LS‑B11793

IHC‑plus™ Polyclonal Rabbit anti‑Human APG5 / ATG5 Antibody (aa1‑30, IHC, IF, WB) LS‑B11793

Note: This antibody replaces LS-C156611
Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B11793-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
APG5 / ATG5 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ATG5 antibody LS-B11793 is an unconjugated rabbit polyclonal antibody to ATG5 (APG5) (aa1-30) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APG5 / ATG5
Synonyms
ATG5 | APG5 | APG5-LIKE | APG5L | Apoptosis specific protein | Apoptosis-specific protein | Autophagy related 5 | HAPG5 | ASP | Autophagy protein 5
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa1-30
Specificity
This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG5.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG5 / ATG5
Q9H1Y0 NM_004849 NP_004840.1

Validation

APG5 / ATG5 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

APG5 / ATG5 Antibody - Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ APG5 / ATG5 Antibody (aa1-30) for IHC, IF/Immunofluorescence, WB/Western LS-B11793 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/16/2024