Target
Human ALOX15B / 15-LOX-2
Synonyms
ALOX15B | 15S-lipoxygenase | 15-lipoxygenase 2 | 15-LOX-2 | 15-LOX-B | Arachidonate 15-lipoxygenase B | Arachidonate 15-lipoxygenase 2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Human 15-lipoxygenase-2 (15-LO-2) amino acids 161-179 (CLDEKTVEDLELNIKYSTA). Percent identity by BLAST analysis: Human (100%); Gorilla (94%).
Specificity
Human 15-lipoxygenase-2 (15-LO-2) amino acids 161-179 (CLDEKTVEDLELNIKYSTA. Antibody did not cross-react with 15-LO-1 in Western testing using recombinant protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B1588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1588 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.4, 50% glycerol, 0.1% BSA, 0.02 % sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee