Human ADGRA3 / GPR125

Goat

Human, Monkey, Mouse, Bat, Bovine, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence CTNKSASSFKNS, from the internal region of the protein sequence according to NP_660333.2.

aa1098-1109

Human GPR125.

• IHC
• IHC - Paraffin (3.75 µg/ml)
• Western blot (0.5 - 1.5 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:8000)

Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2). Recommended concentration: 0.5-1.5 ug/ml. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q8IWK6 NCBI: AY181243 AAO27355.1