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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3454-200 | 200 µl | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human ABCB5 Antibody (aa1‑30, IHC, WB) LS‑B3454
IHC‑plus™ Polyclonal Rabbit anti‑Human ABCB5 Antibody (aa1‑30, IHC, WB) LS‑B3454
Note: This antibody replaces LS-C100370
Antibody:
ABCB5 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ABCB5 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
ABCB5 antibody LS-B3454 is an unconjugated rabbit polyclonal antibody to human ABCB5 (aa1-30). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human ABCB5
Synonyms
ABCB5 | ABCB5alpha | ABCB5 P-gp | ABCB5beta | ATP-binding cassette protein | EST422562 | P-glycoprotein ABCB5
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa1-30
Specificity
This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
- Flow Cytometry (1:10 - 1:50)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3454 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3454 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ABCB5
Validation
Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ABCB5 Antibody (aa1-30) for IHC, WB/Western, Flow LS-B3454 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells. Freeman JB, Gray ES, Millward M, Pearce R, Ziman M. Journal of translational medicine. 2012 10:192.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/4/2024
Date Requested: 12/4/2024
