Human ABCB11 / BSEP

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Synthetic peptide corresponding to aa 705-718 (Y705LVHEPPLAVVDHK718) of human BSEP (bile salt export pump). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%); Marmoset, Dog (93%).

aa705-718

Recognizes human BSEP. Does not cross-react with human P-glycoprotein.

• IHC
• IHC - Paraffin (5 µg/ml)
• ICC
• Western blot

Immunohistochemistry: LS-B2170 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2170 was determined to be 5 ug/ml.Western Blot: 1:50-1:200Immunocytochemistry: Cytospin preparationsOptimal dilutions to be determined by the researcher.

PBS pH 7.2, 0.7% BSA, 100mM sodium azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O95342 NCBI: NM_003742 NP_003733.2